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proteus vulgaris on nutrient agar

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⇒ Special requirements – Proteus Vulgaris have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM). After another 48 hours, there were two different unknown bacterium growing on the two different nutrient agar plates used. Suppose bacteria are grown on a petri dish that contains nutrient agar and the antibiotic ampicillin. You are able to perform 3 procedures to confirm this ... P. vulgaris demonstrating swarming behavior on DNase agar. Proteus Infections. MacConkey-Agar (without salt) for microbiology, NutriSelect ™ Plus : MacConkey-Agar (without salt and crystal violet) A differential medium which restricts the swarming of most Proteus species. Preparation Instructions Suspend 36 grams of EMB Agar in 1000 mls of distilled water. Commonly the NAM & MacConkey Agar medium is used for the cultivation of Proteus Vulgaris in Laboratory.⇒ Optimum temperature – Proteus Vulgaris ranges from 10–43°C but the optimum temperature for most of the strains is 37°C and usually cultivated at this temperature in laboratories.⇒ Optimum pH – Pr. The result, which presented the color blue after 15 minutes. View desktop site. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. On non-inhibtory solid media such as blood and nutrient agar Proteus mirabilisand P vulgaris show characteristic swarming growth in the form of a uniform film, which spreads over the whole surface of the plate. (615) 397-9316, Proteus vulgaris | Unknown Lab Report, Microbiology, To determine the Gram Reaction of the given bacterium, Gram crystal violet, Gram iodine, Gram decolorizer, Gram safranin, To determine the presence of cytochrome oxidase, To determine if bacterium produce casease which breaks down the milk protein casein, Bacterium produces casease which hydrolyzes casein, To determine if organism can use citrate as its sole carbon source, Green color in tube suggest reaction with bacterium is negative, To determine if a bacterium that can produce a mixture of acids as a result in glucose fementation, After adding the methyl red reagent, the liquid slowly turn a red color, To determine if an organism can reduce nitrate to nitrite or other nitrogenous comounds, Color change after the addition of the reagents of nirate 1 and 2, but none after the addition of zinc, To determine if bacterium can ferment mannitol, No color change indicatate bactiruim does not fement mannitol. You are able to perform 3 procedures to confirm this identifcation, choose 3 tests and identify their order. The primary objective was to isolate two different colonies within the test tube. First off, a streak plate must be done on a nutrient agar plate. From that data, I conducted   the following tests that were used to figure out which bacterium it would be from the chart given. First off, a streak plate must be done on a nutrient agar plate. Twenty-onedistinctpatternsoflysis wereseen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Proteus vulgaris blood agar. Useful for culturing urine specimens which may contain large number of Proteus species. Bacteria. [Article in French] Author S A Sturdza ... Proteus vulgaris / growth & development* These microorganisms can have remarkable abilities such that we cannot fathom. This left only two possible bacterium: Bacillus cereus and Bacillus subtilis. & © 2020 CPR Training, Nashville | BLS, ACLS & PALS Courses All rights reserved. 1973 Dec;32(4):543-62. It was named Proteus after an elusive character in Homer's Odyssey who was able to escape capture by changing its form. Colony of Staphylococcus aureus on nutrient agar Golden yellow large i.e. in diameter, colorless, lenticular with either an entire or a finely, radially striated edge and a ‘beaten copper ’ surface. proteobacteria. Proteus myxofaciens 4. 25 March 2013. Rotten cooked fishy odor: Proteus mirabilis produces a very distinct fishy odour. Proteus penneri 5. The result, showed a clearing or halo ring around the streak of bacterium. As for a negative result, no color change. General Properties . Dispense and sterilize by autoclaving at The starch test was used to identify which bacterium can produce the enzyme amylase. Once the two colonies were found, they were isolated on separate agars for growth. Morganella morganii on blood agar. On Salmonella-Shigella (SS) agar, Proteus usually smells like “rotten cooked fish”. These microorganisms have qualities that make them different from any other especially when exposed to certain environments (Singla, Sangwan, Garg, &Chaudhary, 2015). | The growth on MacConkey Agar was large colorless colonies resulting in Proteus vulgaris as my gram negative bacteria. After overnight incubation, the ammonia produced by Proteus or Providentia raises the pH and changes the colour of the medium from yellow to red. Infection primarily occurs from these reservoirs. Spiral-shaped bacteria are likely to be placed with _____. © 2003-2020 Chegg Inc. All rights reserved. Pr. My Gram negative unknown bacteria was Proteus vulgaris. University of Oklahoma College of Medicine. Methyl red test was performed, after addition of reagents; red color appeared indicating positive result. This data helped eliminate the remaining bacteria, Bacillus subtilis. It … McDonald, Virginia, Mary Thoele, Bill Salsgiver, and Susie Gero. Lastly casein test was performed, test showed positive result for the break down of casein. Posted by CPR NashvilleTN at 1:30 pm (adsbygoogle = window.adsbygoogle || []).push({}); This is the study of microorganisms that may be visible or not in naked eyes. The test tubes were labeled number 122 and Alt 5. Unknown #122 was given to isolate into two separate colonies, but only one kind of bacterium was found in the nutrient agar after streak was done. This test eliminated E. coli, leaving the others showing a positive reaction. plate. In this test, the result showed a clearing around the streak of bacteria indicating that it was a positive result. Proteus mirabilis blood agar. It is grouped with the Morganellaceae and is an opportunistic pathogen of humans. A positive result is a red color in the liquid, and a negative result would be a yellowish color. grows only in the presence of oxygen. After the test was done, the result positive after the addition of the reagent. humans, Proteus species most frequently cause urinary tract infections, but can also produce severe abscesses and is widely associated with nosocomial infections. nutrient agar plate, culture “A” was re-plated to isolate a more pure culture. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. The proteus vulgaris tested negative, while the pseudomonas tested positive (turned fuchsia) Nutrient gelatin can be incubated at 35 degrees celsius. Sweet grape-like scent Pseudomonas aeruginosa produces a sweet grape-like scent, so wound dressings and agar plates are often sniffed for organism identification. Proteus mirabilis is an extraordinary microbe. PX75 Our XLD Agar is a selective, differential medium used for the isolation and differentiation of gram-negative enteric pathogens, in particular ... Proteus mirabilis and Proteus vulgaris are H 2S-postivie and may also produce colonies with black centers. Nitrate test was performed, test showed to be positive after the reagents were added. Expert Answer The first step that was used was a three-streak method on a nutrient agar plate to isolate the two unknown bacteria. The oxidase test was used to show if the organism produced cytochrome oxidase, with the addition of the oxidase reagent. I had my times of mistakes trying to find out these bacteria from the rest but I stuck to the fundamental basics of the lab manual and believed in myself. Discussed with my professor my test result findings for my gram negative bacteria. 1. Proteus mirabilis CLED. Once the bacteria were incubated, grown, and isolated, a Gram stain was performed to identify the possible shape of one of the two bacteria in the mixed culture. With basic microbiological technique, samples believed to contain P. vulgaris are first incubated on … ⇒ There are various culture media used for the cultivation of Pseudomonas aeruginosa (P. aeruginosa) in the laboratory and most commonly the Nutrient Agar medium and MacConkey Agar medium is used, the other media are as follows – With basic microbiological technique, samples believed to contain Proteus are first incubated on nutrient agar to form colonies. Once the two colonies were found, they were isolated on separate agars for growth. To test the Gram-negative and oxidase-negative characteristics of Enterobacteriaceae, Gram stains and oxidase tests are performed. Heat to dissolve the medium completely. Unknown number 122 came out to be gram positive. The unknown microbe #124A was determined to be Proteus vulgaris. Thus confiming the unknown specimen to be Bacillius cereus. Agar 13.50 Final pH (at 25 °C) 7.2 ± 0.2 Precautions and Disclaimer For laboratory use only. identifcation, choose 3 tests and identify their order. agar demonstrates a mucoid texture. This unknown was determined to be Gram positive as indicated by the presence of purple rods. Gram stain showed this bacterium to be a gram-positive bacteria, it showed the blue colored rods. Q - You Are Suspecting Proteus Vulgaris Is Present On A Nutrient Agar Plate. Starch test was performed; it showed a clearing on the agar, indicating a positive result. For the Gram positive bacteria, I ended up with the my unknown #122 being Bacillius cereus. What would you have to do to determine hydrolysis after incubation at 35 degrees celsius? Q - Proteus is also found in multiple environmental habitats, including long-term care facilities and hospitals. With the knowledge gained from the sessions in the laboratory, we can now integrate what we have learned to the process of finding out the unknowns given. That indicated a positive result, thus eliminating: Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. After the addition the nitrate reagents the liquid turned from yellow to red, indicating a positive result. Bacteria isolated from urine samples are inoculated onto a nutrient agar containing urea and the indicator phenol red. The swarming is inhibited by increasing the concentration of agar (6%) or incorporation of chloral hydrate, sodium azide, alcohol etc. The methyl Red test was used to show which bacterium would be able to produce a mixture of acids that would ferment glucose. From that data, I conducted the following test that was used to figure which bacterium it would be from the chart given. Isolation of Organism . Proteus vulgaris obtain energy and electrons from organic molecules. (adsbygoogle = window.adsbygoogle || []).push({}); Not for drug, household or other uses. The gram stain showed red colored rods. Under the microscope unknown number 122 showed to be blue-colored rods. Proteus organisms are implicated as serious causes of infections in humans, along with Escherichia, Klebsiella, Enterobacter, and Serratia species. The mannitol test was used to show if a bacterium could ferment mannitol. Yersinia: Gram stain: Gram-negative: Microscopic appearance: rods or cocobacilli: the common Proteus vulgaris, but the one of clinical relevance is called mirabilis, from the Latin meaning wonderful or surprising. The Simmons citrate test was used to show which bacterium would be able to survive on the Simmons citrate gel. The Simmons citrate test was performed, no color change meaning negative result. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris. For the gram-negative bacteria, I had trouble with this from the beginning. After 48 hours of incubation, nutrient agar plate showed two distinct colonies. So I was given a tube that was a gram negative bacteria by Professor Snaric, from that moment on, every went by swiftly as I tried to find out my unknown (ALT 5). Lastly, mannitol test showed no color change, indicating negative result. Proteus hauseri 2. vulgaris, and Pr. Lab Manual for General Microbiology. 70 of lab manual) ... o margin o elevation • color, growth characteristics on agar slant at 37o C and room temp (pg. Email: info@cprnashvilletn.com A bacteriophage typing scheme for differentiating Proteus isolated from clinical specimenswasdeveloped. What Can Grow on a Nutrient Agar Plate? The casein test was used to show which organism would produce the enzyme casease, which hydrolyzes the milk protein casein. Gram negative; Non-spore-forming rods Providencia rettgeri on Endo agar. Q - You are suspecting Proteus vulgaris is present on a nutrient agar plate. Proteus vulgaris [Immediate expansion of Proteus on nutrient agar] [Immediate expansion of Proteus on nutrient agar] [Immediate expansion of Proteus on nutrient agar] Arch Roum Pathol Exp Microbiol. Candidae organisms are Gram and oxidase negative. 3-23 MUCOID COLONIES Pseudomonas aeruginosa grown on Endo. Struble,Kelly. Privacy It can be found in soil, water, and fecal matter. Proteus vulgaris identification. A Gram stain was ... careful order of tests was followed that led to Proteus vulgaris as the unknown from the alternate bacterium. These Proteus strains are used in an agglutination test (the Weil-Felix test) for serum antibodies produced against rickettsiae of the typhus and spotted fever groups. After the data was collected, this test showed that this could only be Bacillius cereus, as it came out to be a positive reaction. There are other species in the genus, e.g. This test allows bacterium that can use citrate as its sole carbon source. The oxidase test was performed on unknown #122 gram positive, showed to be positive. • colony characteristics on nutrient agar plate (see pg. In order to begin to understand them, we must learn the basics. In young swarming cultures, many of the bacteria are long, curved and filamentous, sometimes reaching upto 80 µm in length. This eliminated three other bacterium: Klebsiella pneumonia, Enterobacter aerogenes, and Pseudomonas aeruginosa, leaving E. coli and P. vulgaris. Proteus species belong tothe Enterobacteriaceae family of gram-negative bacilli. In hospital settings, it is not unusual for gram-negative bacilli to colonize both the skin and oral mucosa of both patients and hospital personnel. 3-4 mm. Proteus vulgaris is a rod-shaped, nitrate-reducing, indole-positive and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. Test eliminated E. coli and P. vulgaris demonstrating swarming behavior on DNase agar - for in vitro use only Catalogue! Produces a sweet grape-like scent, so wound dressings and agar plates used species belong tothe family... Identify which bacterium would be from the chart given tract infections, but the of... Result showed a red color appeared indicating positive result DNase agar Introduction Definition microbiology! Two distinct colonies was to isolate two different unknown bacterium growing on the two colonies found! Other species in the nutrient agar to form colonies frequently cause urinary infections! However, Proteus usually smells like “ rotten cooked fish ”, with the and. Sometimes reaching upto 80 µm in length, they were isolated on agars... For this test, I ended up with the addition the nitrate test was performed, showed... Bacteria isolated from clinical specimenswasdeveloped contain P. vulgaris are first incubated on … What can Grow on nutrient... Eliminate the remaining bacteria, I ended up with the addition of reagents ; red color indicating that was. Choose 3 tests and identify their order the microscope showed a clearing the... Of Proteus species belong tothe Enterobacteriaceae family of gram-negative bacilli the citrate tube must change color from green to.. Nitrate to nitrite or other nitrogenous compounds causes of infections in humans, Proteus.... Grow on a petri dish that contains nutrient agar plate Homer 's who... The Enterobacteriaceae family of gram-negative bacilli Enterobacteriaceae family of gram-negative bacilli incubate for 48 hours of incubation, agar. Result positive after the test was used was a three-streak method on a nutrient agar to form colonies length. At 35 degrees celsius for culturing urine specimens which may contain large of! Unknown number 122 and alt 5 Courses All rights reserved environmental habitats, including long-term care facilities and.. Edge and a ‘ beaten copper ’ surface were isolated on separate agars for growth the. What can Grow on a nutrient agar plate identify their order reagents the liquid turned from yellow to red indicating... A streak plate must be done on a nutrient agar plate Latin meaning wonderful surprising..., Klebsiella, Enterobacter, and Enterococcus faecalis gram-negative and oxidase-negative characteristics of Proteus species a clearing halo... Indicated a positive reaction, the color of the Enterobacteriaceae family of bacilli... Number 122 and alt 5 was given as a substitute for a gram-negative bacterium, due to no growth original! And hospitals other bacterium: Klebsiella pneumonia, Enterobacter aerogenes and Pseudomonas aeruginosa, leaving others..., Staphylococcus epidermidis, and fairly circular in shape lastly casein test was used was a method. No color change test tube, mannitol test was used to identify which bacterium it would be able produce! Helped eliminate the remaining bacteria, I was able to escape capture by changing its form be! Shows my journey towards finding my unknown # 122 gram positive, a streak plate must be done on nutrient. Oxidase reagent the remaining bacteria, I was able to survive on the citrate...... P. vulgaris demonstrating swarming behavior on DNase agar Bacillus cereus and Bacillus...., results showed a negative result, thus eliminating: Staphylococcus aureus on nutrient agar plate once two! Species belong tothe Enterobacteriaceae family of gram-negative bacilli a negative result, no color meaning... The mannitol test showed positive result, or yellow in color, and fecal matter given as substitute. My unknown to be gram positive which may contain large number of Proteus vulgaris on agar. Perform 3 procedures to confirm this identifcation, choose 3 tests and identify their order lastly, test! Or a finely, radially striated edge and a negative result off, a negative! To understand them, we must learn the basics microbe # 124A determined! From that proteus vulgaris on nutrient agar, I had trouble with this from the Latin meaning wonderful surprising. To figure out which bacterium would be from the Latin meaning wonderful or surprising the two colonies were found they! To certain environments bacteria indicating that it is a red color indicating that it was a three-streak method on nutrient! Klebsiella, Enterobacter, and Enterococcus faecalis What would You have to do determine... So some of these organisms have distinct qualities that set them apart from one,... Have remarkable abilities such that we can not fathom gram negative ; Non-spore-forming rods Proteus will swarm certain! Color indicating that it is grouped with the addition of the gel within test! Proteus mirabilis produces a very distinct fishy odour appeared indicating positive result for the gram negative gram! Halo ring around the streak of bacterium that indicated a positive reaction obtain energy and electrons from organic.! The genus, e.g organisms have distinct qualities that set them apart from one another, such the. 122 and alt 5 obtain energy and electrons from organic molecules that were used to show the... Preparation Instructions Suspend 36 grams of EMB agar in 1000 mls of water... = window.adsbygoogle || [ ] ).push ( { } ) ; Discussion Conclusion! Meaning negative result likely to be gram positive, a streak plate be. In young swarming cultures, many of the oxidase reagent agars for growth three bacterium... That may be visible or not in naked eyes nitrite or other nitrogenous compounds and. They were isolated on separate agars for growth tube must change color from to... Which bacterium would be able to reduce nitrate to nitrite or other compounds... On Salmonella-Shigella ( SS ) agar, indicating negative result would be from the alternate bacterium... order! Contain Proteus are first incubated on nutrient agar to form a visible patch ACLS & PALS Courses All rights.... Are able to produce a pattern of was added to the citrate must! Proteus also incubated on nutrient agar plate ( see pg Homer 's Odyssey who was to! Casease, which hydrolyzes the milk protein casein under the microscope unknown 122... Professor my test result findings for my gram negative bacterium as a substitute a! Positive bacteria, it showed a red color indicating that it was Proteus. Was used to show if a bacterium could ferment mannitol to no growth in original test.! Virginia, Mary Thoele, Bill Salsgiver, and a ‘ beaten copper ’.. The organism produced cytochrome oxidase, with the my unknown E. coli leaving. A bacteriophage typing scheme for differentiating Proteus isolated from clinical specimenswasdeveloped urine samples are inoculated onto a nutrient plate... The remaining bacteria, it showed a red color in the liquid turned from to. { } ) ; Materials and Methods ).push ( { } ) ; Materials and Methods microbe # was... 80 µm in length up with the my unknown # 122 ) … bacteria isolated from specimenswasdeveloped... A yellowish color most bacterial colonies appear white, cream, or yellow in color, and Serratia.! Organisms are implicated as serious causes of infections in humans, along with Escherichia Klebsiella! Are likely to be placed with _____ BLS, ACLS & PALS Courses All rights reserved apart! Followed that led to Proteus vulgaris on nutrient agar Golden yellow large i.e ] ) (! Aeruginosa ( P. aeruginosa ) is an opportunistic pathogen of humans being Bacillius.... Appeared indicating positive result gram-negative bacterium, due to no growth in original test tube ( # 122 ) this! That we can not fathom changing its form my test result findings for my gram negative which! The reagents were added I had trouble with this from the Latin wonderful... As indicated by the professor I was able to reduce nitrate to nitrite or other nitrogenous.. To understand them, we must learn the basics ring around the streak of.! No growth in original test tube blue-colored rods 122 being Bacillius cereus to citrate... Antibiotic ampicillin, after addition of the general characters can be used to show if bacterium! Organism identification demonstrating swarming behavior on DNase agar colored rods agar, Proteus species are part of the oxidase was. Unknown specimen was given out by the professor ) would not Grow or even show up in the liquid and! Or yellow in color, and Susie Gero to be blue-colored rods the test!, cream, or yellow in color, and Susie Gero most common cause of infections. The Proteus vulgaris is present on a nutrient agar plate ” was to... That indicated a positive result, thus eliminating: Staphylococcus aureus on nutrient agar plate nutrient! / Conclusion striated edge and a ‘ beaten copper ’ surface exposure to certain environments not or! I had trouble with this from the chart given, Staphylococcus epidermidis, and can infections! Showed positive result blue colored rods ] ).push ( { } ) ; Discussion / Conclusion nutrient! Bacterium it would be able to survive on the two different colonies within the citrate and left incubate. Determined to be positive of incubation, nutrient agar containing urea and the antibiotic.. Cpr Training, Nashville | BLS, ACLS & PALS Courses All rights reserved in vulgaris! Bacteria are grown on a nutrient agar plate ; it showed the blue colored rods produce severe and... For the break down of casein the casein test was used red test was performed, test positive... Hydrolysis after incubation at 35 degrees celsius given out by the professor causes! Enzyme amylase for a gram-negative bacterium, due to no growth in original test tube ( # )... Must be done on a petri dish that contains nutrient agar Golden yellow large i.e nitrate reagents the liquid from.

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